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System Requirements

ComponentMinimumRecommended
RAM4 GB16 GB or more for large whole-slide images
CPU2 cores, 64-bit x86-64 (Windows/Linux) or Apple Silicon arm64 (macOS)4+ cores for faster parallel pipeline execution
Free disk space~5 GBMore if you plan to store multiple AI model files locally
Display1280×8001920×1080 or larger
Operating systemNotes
WindowsWindows 10 or later, x86-64
LinuxA modern 64-bit distribution (glibc 2.31 or newer - e.g. Ubuntu 20.04+, Debian 11+, Fedora 34+); see the Installation page for required GUI libraries
macOSmacOS 13 (Ventura) or later, Apple Silicon (arm64)

AI segmentation steps (Stardist, UNet, Cellpose) run on CPU by default and work on every supported platform. For significantly faster inference, download a CUDA build (Windows or Linux only - see Downloads) if your machine has:

ComponentRequirement
GPUAn NVIDIA GPU with compute capability 6.0 or higher
DriverAn NVIDIA driver new enough to support CUDA 12.x
VRAM4 GB minimum; more for large images or batches of high-resolution tiles

If you don’t have a CUDA-capable GPU, install the regular CPU build - AI segmentation still works, just slower.

EVAnalyzer streams large images in tiles rather than loading them fully into memory, but very large whole-slide or multi-well-plate datasets still benefit from more RAM and a faster disk (SSD recommended) - see Image Formats for how tiled/pyramidal formats are handled.