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Analyzed: the same image with EVAnalyzer's nucleus and cell segmentation overlay Raw: unprocessed fluorescence microscopy image before analysis Raw Analyzed

Enhanced Visual Analyzer (EVAnalyzer) High-performance bioimage analysis featuring a multi-channel viewer and customizable pipelines.

EVAnalyzer is the next-generation successor to the original EVAnalyzer ImageJ plugin. Rebuilt for modern workflows, it pairs a high-performance image viewer with a fully configurable, multi-step analysis pipeline builder. While adaptable to any image format, EVAnalyzer is optimized for complex datasets, including fluorescence microscopy and high-content, whole-slide screening data. Designed for scale, the platform efficiently batch-processes thousands of images simultaneously, storing all results in a structured local database for seamless export and downstream analysis. See About for the full history.

CZIND2LIFVSIOME-TIFFBIG-TIFFSLDSCN+ 30 more

Installation

Download and install EVAnalyzer on Linux, Windows, or macOS. Get started →

First Steps

Create your first project and run your first analysis. Learn more →

Tutorials

Step-by-step guides for spot counting, colocalization, and more. View tutorials →

The same zoomed-in pipeline preview and segmentation overlays in dark modeEVAnalyzer's pipeline editor in light mode, zoomed in on detected nuclei with segmentation overlays over a multi-channel fluorescence microscopy imageLightDark

Configure multi-step pipelines and preview segmentation results live against your raw image data - in light or dark mode.

Multiple pipelines

Create as many independent image processing pipelines as your experiment needs - one per channel, or chained together for complex multi-step workflows.

40+ file formats

CZI, ND2, LIF, VSI, OME-TIFF, SLD, SCN, and more via Bio-Formats, with full multi-channel support out of the box.

Whole-slide image support

Large pyramidal, tiled whole-slide images are streamed and analysed tile by tile automatically - no manual downsampling required.

Z-stacks & time-lapse

Intensity projections (Max, Min, Avg, Sum, Middle) or per-frame processing across Z-stacks and time-lapse (T-stack) sequences.

Live multi-channel viewer

Per-channel brightness/contrast, visibility toggles, and colour assignment, with a live pipeline preview that updates as you tune each step.

Batch processing at scale

Queue and process thousands of images unattended - the same pipeline runs consistently across an entire dataset, storing results as it goes.

Built in Rust

EVAnalyzer is written in Rust rather than Python, for native-code performance and memory safety across large batch runs.

Classical & AI segmentation

Combine classical algorithms (Threshold, Watershed, Edge Detection) with AI-based object detection (Stardist, UNet, Cellpose) in the same pipeline - or import a published bioimage.io model to auto-configure one.

See the full feature list →