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Image Cache

The Image Cache command saves or loads an intermediate image to/from a named cache slot. Cache slots are shared across all pipelines within a single image analysis run.

Pipelines in EVAnalyzer normally run independently, each seeing only its own image data - Image Cache is the one mechanism that lets one pipeline hand a processed image to another, so a channel-specific preprocessing result (a background estimate, a mask) can be reused elsewhere instead of being recomputed.

A checkpoint stored by one pipeline and loaded by another

ParameterDescription
ModeStore - save the current image to the cache; Load - replace the current image with a cached image
AddressThe cache slot identifier

Use Image Cache when you need to:

  • Re-use an intermediate processing result in a later pipeline step or a different pipeline.
  • Pass a pre-processed image to Image Math as the second operand.

Example: background-subtracted signal available for measurement

Section titled “Example: background-subtracted signal available for measurement”
  1. Pipeline A: process the DAPI channel → Store to cache slot dapi_bg.
  2. Pipeline B: after thresholding the Cy5 channel, use Image Math with the dapi_bg cache to create a masked image.

Cache contents are discarded at the end of each image’s processing. They do not persist between images.