{
  "schemaVersion": 2,
  "meta": {
    "name": "Yeast cells brightfield",
    "shortDescription": "Yeast cells brightfield",
    "description": "Segments yeast cells from a brightfield image using contrast enhancement, Gaussian blur, Canny edge detection, manual thresholding and a Voronoi tessellation around detected cell centers. The output object class can be remapped after import (default class id used during conversion: 4). Approximation notes: Voronoi centers reference 'M01' (a Hough-transform output label) which has no equivalent class; centers set to UNSET and should be remapped manually $houghTransform has no equivalent in the new schema and was approximated with ConnectedComponents",
    "authors": [
      "Melanie Schuerz"
    ],
    "authorOrganization": "University of Salzburg",
    "creationTime": "2026-06-11T00:00:00Z"
  },
  "steps": [
    {
      "enabled": true,
      "command": {
        "type": "INTENSITY_TRANSFORMATION",
        "mode": "MANUAL",
        "contrast": 0.8,
        "brightness": 0.0
      }
    },
    {
      "enabled": true,
      "command": {
        "type": "GAUSSIAN_BLUR",
        "kernelSize": 5,
        "sigma": 1.0
      }
    },
    {
      "enabled": true,
      "command": {
        "type": "EDGE_DETECTION_CANNY",
        "kernelSize": 3,
        "thresholdMin": 0.0196078431372549,
        "thresholdMax": 0.23529411764705882
      }
    },
    {
      "enabled": true,
      "command": {
        "type": "THRESHOLD",
        "thresholds": [
          {
            "method": {
              "type": "MANUAL"
            },
            "minThreshold": 4000.0,
            "maxThreshold": 65535.0,
            "unit": "BIT",
            "objectClassId": 1
          }
        ]
      }
    },
    {
      "enabled": true,
      "command": {
        "type": "CONNECTED_COMPONENTS"
      }
    },
    {
      "enabled": true,
      "command": {
        "type": "VORONOI",
        "centers": "UNSET",
        "centerFilterClasses": [],
        "mask": "UNSET",
        "maskFilterClasses": [],
        "outputClass": {
          "VALID": 4
        },
        "unit": "PIXELS",
        "maxRadius": 30.0,
        "excludeAreasAtTheEdges": false,
        "excludeAreasWithNoCenter": false
      }
    }
  ]
}